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Proceedings Paper

NIR-laser tissue welding in an in vivo guinea pig animal model
Author(s): Vidyasagar Sriramoju; Howard E. Savage; A. Katz; Rahul Chakraverty; Yuri Budansky; Rakhi Podder; Naghmeh Davatgarzadeh; Uladzimir Kartazayev; Richard B. Rosen; R. R. Alfano
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Paper Abstract

Near infrared laser tissue welding (LTW) is achieved by subjecting the closely approximated surgically incised tissues to a laser beam at a wavelength that is absorbed by water in the tissue. Full thickness welds are accomplished with optimum laser power and penetration depths appropriate for the thickness of welded tissues. No extrinsic cross-linking or bonding materials are used. The absorbed laser energy increases the entropy of collagen in the tissue. In LTW, tissue water temperatures transiently rises to approximately 60° C, causing partial denaturing of collagen and other structural proteins due to breaking of hydrogen bonds, electrostatic interactions and some interchain covalent bonds for a short duration of time. This is followed by cross linking of proteins on either side of weld line, with reformation of the above mentioned bonds as the tissue cools, resulting in the formation of water tight full thickness welds. In this study, a cw fiber laser emitting at 1455 nm, corresponding to absorption by a water vibrational overtone, is used for in vivo LTW of surgical incisions made in the skin of guinea pigs under general anesthesia. The tensile strength and healing rates of the welded incisions are compared to suturing of similar incisions. Laser parameters, including power, scanning rates, exposure area, and exposure duration, are optimized to reduce thermal damage while maintaining tensile strength.

Paper Details

Date Published: 11 March 2008
PDF: 4 pages
Proc. SPIE 6854, Optical Interactions with Tissue and Cells XIX, 685405 (11 March 2008); doi: 10.1117/12.760044
Show Author Affiliations
Vidyasagar Sriramoju, City College of New York (United States)
Howard E. Savage, New York Eye and Ear Infirmary (United States)
A. Katz, City College of New York (United States)
Rahul Chakraverty, City College of New York (United States)
Yuri Budansky, City College of New York (United States)
Rakhi Podder, City College of New York (United States)
Naghmeh Davatgarzadeh, City College of New York (United States)
Uladzimir Kartazayev, City College of New York (United States)
Richard B. Rosen, New York Eye and Ear Infirmary (United States)
R. R. Alfano, City College of New York (United States)


Published in SPIE Proceedings Vol. 6854:
Optical Interactions with Tissue and Cells XIX
Steven L. Jacques; William P. Roach; Robert J. Thomas, Editor(s)

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