Share Email Print
cover

Proceedings Paper

Polychromatic flow cytometry with an avalanche photodiode array
Author(s): William G. Lawrence; Gyula Varadi; Gerald Entine; Edward Podniesinski; Paul K. Wallace
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Multi parameter flow cytometry enables detailed identification of cell type and function based on fluorescence of antibody conjugated dye labels. Current instruments use photomultiplier tube detectors to measure up to eight fluorescent labels from a single excitation source. We demonstrate polychromatic flow cytometry using a 14-element avalanche photodiode (APD) array coupled with a dispersive optical grating. Forward scatter, side scatter, and 14 fluorescence channels over the 530 to 800 nm spectral range are recorded using a 16 channel electronics console for simultaneous event capture. The APD detector elements have a working spectral range from 400 nm to 1050 nm. Results are presented for flow cytometry measurements of Spherotech UltraRainbow test beads, quantum dot labeled polystyrene spheres, and cells with antibody conjugated dye labels. The flow cytometry test bead measurements illustrate the sensitivity and spectral resolution of the APD detector array. The application of the instrument is demonstrated by identifying CD4 positive lymphocyte populations in normal human whole blood samples.

Paper Details

Date Published: 29 February 2008
PDF: 10 pages
Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 68591L (29 February 2008); doi: 10.1117/12.758959
Show Author Affiliations
William G. Lawrence, Radiation Monitoring Devices, Inc. (United States)
Gyula Varadi, Radiation Monitoring Devices, Inc. (United States)
Gerald Entine, Radiation Monitoring Devices, Inc. (United States)
Edward Podniesinski, Roswell Park Cancer Institute (United States)
Paul K. Wallace, Roswell Park Cancer Institute (United States)


Published in SPIE Proceedings Vol. 6859:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

© SPIE. Terms of Use
Back to Top