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Proceedings Paper

Side-polished fiber immunosensor based on surface plasmon resonance for detection of Legionella pneumophila
Author(s): Yu-Chia Tsao; Yi-Wen Yang; Woo-Hu Tsai; Tsong-Rong Yan
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Paper Abstract

Side-polished fiber immunosensor based on surface plasmon resonance (SPR) onto self-assembled protein A layer was proposed for the detection of Legionella pneumophila. A self-assembled protein A layer on gold (Au) surface was fabricated by adsorbing a mixture of 11-mercaptoundecanoic acid (MUA) and activated by N-Ethyl-N'-(3-dimethylaminopropyl) carbodiimide/ N-Hydroxysuccinimide (EDC/NHS). The formation of self-assembled protein A and gold layer on side-polished surface and the binding of antibody and antigen in series were confirmed by SPR response on spectrum. The binding protein A layer can improve the sensitivity, which indirectly supports the configurations that antibody layer is immobilized on the binding protein A layer with a well-ordered orientation. The surface morphology analyses of self-assembled protein A layer on Au substrate and monoclonal antibody against L. pneumophila immobilized on protein A were demonstrated by SPR dip shifts on optical spectrum analyzer. The SPR fiber immunosensor for detection of L. pneumophila was developed and the detection limit was 10 CFU/ml with the SPR dip shift in wavelength from 1070 to 1105nm. The current fabrication technique of a SPR immunosensor using optical fiber for the detection of Legionella pneumophila could be applied to construct other biosensor.

Paper Details

Date Published: 7 February 2008
PDF: 8 pages
Proc. SPIE 6852, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VIII, 685206 (7 February 2008); doi: 10.1117/12.758498
Show Author Affiliations
Yu-Chia Tsao, Forward Electronics Co., Ltd. (Taiwan)
Yi-Wen Yang, Forward Electronics Co., Ltd. (Taiwan)
Woo-Hu Tsai, Tatung Univ. (Taiwan)
Tsong-Rong Yan, Tatung Univ. (Taiwan)


Published in SPIE Proceedings Vol. 6852:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VIII
Israel Gannot, Editor(s)

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