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Proceedings Paper

UV-B radiation induced effects on human T-lymphocytes
Author(s): Gianfranco Giubileo; Agostina Congiu Castellano; Silvia Gaudenzi; Paola Grimaldi; Deleana Pozzi; Adriana Puiu
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Paper Abstract

The reactive oxygen species (ROS) produced inside cells by UV-B radiation may induce apoptosis, a process that realizes the programmed death of cells. In the present paper the UV induced damage was studied in a human T-Lymphocyte cell line (the Jurkat line) by mean of IR laser photoacoustic spectroscopy (PAS) and by Fourier Transform Infrared Spectroscopy (FTIR) combined with biological assays based on flow cytometry. The apoptosis was induced in vitro in the Jurkat samples by exposition to UV-B radiation with a dose of 310 mJ/cm2. PAS measurements were performed through a 10W c.w. CO2 laser based optical system realised at ENEA Molecular Spectroscopy Laboratory in Frascati (Italy).

Paper Details

Date Published: 11 September 2007
PDF: 10 pages
Proc. SPIE 6785, ROMOPTO 2006: Eighth Conference on Optics, 67851H (11 September 2007); doi: 10.1117/12.757866
Show Author Affiliations
Gianfranco Giubileo, ENEA (Italy)
Agostina Congiu Castellano, Univ. di Roma La Sapienza (Italy)
Silvia Gaudenzi, Univ. di Roma La Sapienza (Italy)
Paola Grimaldi, Univ. di Roma La Sapienza (Italy)
Deleana Pozzi, Univ. di Roma La Sapienza (Italy)
Adriana Puiu, National Institute for Laser, Plasma and Radiation Physics (Romania)

Published in SPIE Proceedings Vol. 6785:
ROMOPTO 2006: Eighth Conference on Optics
Valentin I. Vlad, Editor(s)

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