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Proceedings Paper

Quantum dot-based western blot for sensitive detection of pig serum antibody to actinobacillus pleuropneumoniae
Author(s): Ana Cişmileanu; Cornelia Sima; Constantin Grigoriu
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Paper Abstract

A quantum dot - immunoglobulin conjugate specific for pig IgG, was obtained by carbodiimide chemistry. We used a Western blot technique for detecting specific antibodies against Actinobacillus pleuropneumoniae (A. pp), which cause porcine pleuropneumonia. The antigen used in this technique was Apx haemolysin which is an important virulence factor of A. pp and it induces protective immunity in vaccined pigs. The detection on Western blot membrane was possible at 1/50 dilution of quantum dot conjugate at a dilution of pig serum till 1/6400. The results for pig serum demonstrated a higher sensitivity of QD-based Western blot technique for the presence of antibodies specific for Apx haemolysin in comparison with similar classical techniques (with coloured substrate for enzyme present in secondary antibody conjugate).

Paper Details

Date Published: 1 August 2007
PDF: 6 pages
Proc. SPIE 6785, ROMOPTO 2006: Eighth Conference on Optics, 67850J (1 August 2007); doi: 10.1117/12.756829
Show Author Affiliations
Ana Cişmileanu, Pasteur Institute (Romania)
Cornelia Sima, National Institute for Laser, Plasma and Radiation Physics (Romania)
Constantin Grigoriu, National Institute for Laser, Plasma and Radiation Physics (Romania)

Published in SPIE Proceedings Vol. 6785:
ROMOPTO 2006: Eighth Conference on Optics
Valentin I. Vlad, Editor(s)

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