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Proceedings Paper

mTHPC-photodynamic therapy induced apoptosis in nasopharyngeal carcinoma cells
Author(s): Christine M. N. Yow; Albert W. N. Leung; Zheng Huang
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Paper Abstract

In this study, the early apoptotic events of mTHPC-medicated photodynamic therapy (PDT) was explored in two human nasopharyngeal carcinoma (NPC) cell lines - NPC/HK1 cells and NPC/CNE2 cells. Cells (5 x 103) were incubated with mTHPC (0.8 μg/ml) in chamber slides for 20 h and subjected to light irradiation at 2 J/cm2 (LD80). Morphologic changes of treated cells were examined at 0- 4 h after the light irradiation by a light microscopy. The early stage of apoptosis was detected by fluorescein-conjugated Annexin V (Annexin V-FITC) assay. Mitochondrial membrane damage and cytochrome c release were determined by flowcytometric analysis. The Bcl-2 expression was measured by Western blot analysis. One hour after mTHPC-mediated PDT, membrane blebbing and cell shrinkage appeared in both HK1 and CNE2 cells. Annexin V-FITC assay showed that a considerable number of HK1 and CNE2 cells became apoptotic at 1 h after PDT. Flowcytometric analysis showed that the cytochrome c was released at 1 h after PDT. The Bcl-2 expression also declined significantly in both cell lines compared to the control groups. mTHPC-mediated PDT can effectively induce apoptotic responses in NPC cells which might be modulated by mitochondrial damage and Bcl-2 inhibition.

Paper Details

Date Published: 18 January 2008
PDF: 6 pages
Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 68261P (18 January 2008); doi: 10.1117/12.756807
Show Author Affiliations
Christine M. N. Yow, Hong Kong Polytechnic Univ. (Hong Kong China)
Albert W. N. Leung, The Chinese Univ. of Hong Kong (Hong Kong China)
Zheng Huang, Univ. of Colorado at Denver and Health Sciences Ctr. (United States)

Published in SPIE Proceedings Vol. 6826:
Optics in Health Care and Biomedical Optics III
Xingde Li; Qingming Luo; Ying Gu, Editor(s)

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