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Proceedings Paper

Alpha-fetoprotein detection by using a localized surface plasmon coupled fluorescence fiber-optic biosensor
Author(s): Ying-Feng Chang; Ran-Chou Chen; Ying-Chang Li; Chih-Jen Yu; Bao-Yu Hsieh; Chien Chou
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Paper Abstract

Alpha-fetoprotein (AFP) detection by using a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor is setup and experimentally demonstrated. It is based on gold nanoparticle (GNP) and coupled with localized surface plasmon wave on the surface of GNP. In this experiment, the fluorophores are labeled on anti-AFP which are bound to protein A conjugated GNP. Thus, LSPCF is excited with high efficiency in the near field of localized surface plasmon wave. Therefore, not only the sensitivity of LSPCF biosensor is enhanced but also the specific selectivity of AFP is improved. Experimentally, the ability of real time measurement in the range of AFP concentration from 0.1ng/ml to 100ng/ml was detected. To compare with conventional methods such as enzyme-linked immunosorbent assay (ELISA) or radioimmunoassay (RIA), the LSPCF fiber-optic biosensor performs higher or comparable detection sensitivity, respectively.

Paper Details

Date Published: 8 January 2008
PDF: 8 pages
Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 68261B (8 January 2008); doi: 10.1117/12.754617
Show Author Affiliations
Ying-Feng Chang, National Yang-Ming Univ. (Taiwan)
Ran-Chou Chen, Taipei City Hospital (Taiwan)
National Yang-Ming Univ. (Taiwan)
Ying-Chang Li, National Central Univ. (Taiwan)
Chih-Jen Yu, National Central Univ. (Taiwan)
Bao-Yu Hsieh, National Yang-Ming Univ. (Taiwan)
Chien Chou, National Yang-Ming Univ. (Taiwan)
National Central Univ. (Taiwan)


Published in SPIE Proceedings Vol. 6826:
Optics in Health Care and Biomedical Optics III
Xingde Li; Qingming Luo; Ying Gu, Editor(s)

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