Share Email Print
cover

Proceedings Paper

Development of human granulocyte morphometric model for scanning flow cytometry
Author(s): Gennady I. Ruban; Svetlana M. Kosmacheva; Natalia V. Goncharova; Valery A. Loiko
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Cell structure, shape, and size distribution are characterized by optical measurement of normal peripheral blood granulocytes in a cell suspension as applied to a cell discrimination by the solution of the direct and inverse light-scattering problems in scanning flow cytometry (SFC). The distribution laws of the cell and nuclei sizes are estimated. A morphometric model of a segmented neutrophilic granulocyte is constructed on the base of the gained data. An interrelation between the cell and nucleus metric characteristics is deduced. The obtained interrelation allows decreasing a number of independent parameters of the cell model to adapt it to the real-time discrimination of the cells. The findings can be used as input parameters for the solution of the direct and inverse light-scattering problems in SFC-dispensing with a costly and time-consuming immunophenotyping of the cells.

Paper Details

Date Published: 7 August 2007
PDF: 6 pages
Proc. SPIE 6734, International Conference on Lasers, Applications, and Technologies 2007: Laser Technologies for Medicine, 67341C (7 August 2007); doi: 10.1117/12.753259
Show Author Affiliations
Gennady I. Ruban, Stepanov Institute of Physics (Belarus)
Svetlana M. Kosmacheva, Ctr. of Hematology and Transfusiology (Belarus)
Natalia V. Goncharova, Ctr. of Hematology and Transfusiology (Belarus)
Valery A. Loiko, Stepanov Institute of Physics (Belarus)


Published in SPIE Proceedings Vol. 6734:
International Conference on Lasers, Applications, and Technologies 2007: Laser Technologies for Medicine

© SPIE. Terms of Use
Back to Top