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Proceedings Paper

New perspectives in protein-based biosensors: the glucokinase from B. stearothermophilus and the odorant-binding protein from C. familiaris as probes for non-consuming analyte sensors
Author(s): Irina M. Kuznetsova; Olga Povarova; Olesya Stepanenko; Konstantin K. Turoverov; Roberta Crescenzo; Antonio Varriale; Maria Staiano; Sabato D'Auria
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Paper Abstract

Glucose sensing and odorant molecules sensing are used as a models to explore the advantages and problems deriving from the use of either enzymes or odorant-binding proteins to develop stable optical biosensors. We report on a novel approach to address the problem of substrate consumption by sensors based on enzymes, namely the utilization of apoenzymes as non-active forms of the protein which are still able to bind the substrate/ligand. We also show studies in which the isolation of an odorant-binding protein from the nose of the dog is used as non-consuming analyte probe for the realization of an integrated optical sensor.

Paper Details

Date Published: 27 July 2007
PDF: 6 pages
Proc. SPIE 6733, International Conference on Lasers, Applications, and Technologies 2007: Environmental Monitoring and Ecological Applications; Optical Sensors in Biological, Chemical, and Engineering Technologies; and Femtosecond Laser Pulse Filamentation, 673318 (27 July 2007); doi: 10.1117/12.753249
Show Author Affiliations
Irina M. Kuznetsova, Institute of Cytology (Russia)
Olga Povarova, Institute of Cytology (Russia)
Olesya Stepanenko, Institute of Cytology (Russia)
Konstantin K. Turoverov, Institute of Cytology (Russia)
Roberta Crescenzo, Consiglio Nazionale delle Ricerche (Italy)
Antonio Varriale, Consiglio Nazionale delle Ricerche (Italy)
Maria Staiano, Consiglio Nazionale delle Ricerche (Italy)
Sabato D'Auria, Consiglio Nazionale delle Ricerche (Italy)


Published in SPIE Proceedings Vol. 6733:
International Conference on Lasers, Applications, and Technologies 2007: Environmental Monitoring and Ecological Applications; Optical Sensors in Biological, Chemical, and Engineering Technologies; and Femtosecond Laser Pulse Filamentation

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