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Proceedings Paper

Effect of LED irradiation on the expression of MMP-3 and MMP-13 in SW1353 cells in vitro
Author(s): Chang-chun Zeng; Zhou-yi Guo; Feng-xue Zhang; Wen-di Deng; Song-hao Liu
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Paper Abstract

Matrix Metalloproteinase (MMP) plays an active role in remodeling cartilage in osteoarthritic cartilage. To find an effective method of prevention of osteoclasia, this in vitro study focuses on the expression of MMP-3 and MMP-13 in the SW1353 cells by LED irradiation. The human chondrosarcoma cell line SW1353 were stimulated with the proinflammatory cytokine IL-1beta or tumor necrosis factor-alpha (TNF-alpha), and were received the irradiation of LED (632nm, 4mW/cm2). The cell count was assessed over a 96-hour period by using Trypan blue dye exclusion assay, and the cell activity was evaluated with a Cell Counting Kit-8 Assays. The subsequent expression of MMP-3 and MMP-13 was quantified. Results of this experiment showed that the cultural cell activity was decreased, and the expression of MMP-3 and MMP-13 was increased by being stimulated with IL-1beta or TNF-alpha. After received LED irradiation, the death rate of cultural cell was increased and the expression of MMP-3 and MMP-13 was decreased significantly. The present study concluded that particular LED irradiation stimulates SW1353 cell proliferation activity and inhibit the MMP-3 and MMP-13 enzymatic activity. These findings might be clinically relevant, indicating that the low power laser irradiation treatment is likely to achieve the repair of articular cartilage in clinic.

Paper Details

Date Published: 1 May 2007
PDF: 6 pages
Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 653423 (1 May 2007); doi: 10.1117/12.741625
Show Author Affiliations
Chang-chun Zeng, Lab. of Photonic Traditional Chinese Medicine, South China Normal Univ. (China)
Zhou-yi Guo, South China Normal Univ. (China)
Feng-xue Zhang, Guangzhou Univ. of Traditional Chinese Medicine (China)
Wen-di Deng, Guangzhou Univ. of Traditional Chinese Medicine (China)
Song-hao Liu, Lab. of Photonic Traditional Chinese Medicine, South China Normal Univ. (China)


Published in SPIE Proceedings Vol. 6534:
Fifth International Conference on Photonics and Imaging in Biology and Medicine

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