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Proceedings Paper

Light-induced damage and its diagnosis in two-photon excited autofluorescence imaging of retinal pigment epithelium cells
Author(s): Danni Chen; Junle Qu; Gaixia Xu; Lingling Zhao; Hanben Niu
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Paper Abstract

In this paper, a novel method for the differentiation of the retinal pigment epithelium (RPE) cells after light-induced damage by two-photon excitation is presented. Fresh samples of RPE cells of pig eyes are obtained from local slaughterhouse. Light-induced damage is produced by the output from Ti: sapphire laser which is focused onto the RPE layer. We study the change of the autofluorescence properties of RPE after two-photon excitation with the same wavelength. Preliminary results show that after two-photon excitation, there are two clear changes in the emission spectrum. The first change is the blue-shift of the emission peak. The emission peak of the intact RPE is located at 592nm, and after excitation, it shifts to 540nm. It is supposed that the excitation has led to the increased autofluorescence of flavin whose emission peak is located at 540nm. The second change is the increased intensity of the emission peak, which might be caused by the accelerated aging because the autofluorescence of RPE would increase during aging process. Experimental results indicate that two-photon excitation could not only lead to the damage of the RPE cells in multiphoton RPE imaging, but also provide an evaluation of the light-induced damage.

Paper Details

Date Published: 1 May 2007
PDF: 7 pages
Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65341L (1 May 2007); doi: 10.1117/12.741597
Show Author Affiliations
Danni Chen, Shenzhen Univ. (China)
Junle Qu, Shenzhen Univ. (China)
Gaixia Xu, Shenzhen Univ. (China)
Lingling Zhao, Shenzhen Univ. (China)
Hanben Niu, Shenzhen Univ. (China)

Published in SPIE Proceedings Vol. 6534:
Fifth International Conference on Photonics and Imaging in Biology and Medicine

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