Share Email Print
cover

Proceedings Paper

Full-field OCT for developmental biology
Author(s): Rui Wang; Ping Xue; Wenjia Bai; Dieyan Chen
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

In this paper, we developed a full-field OCT system using thermal light as the low-coherence light source. A well-known Linnik interferometer configuration was used. Broad spectral width of the thermal light 450-650nm was used to achieve high axial resolution of 1.1&mgr;m in biological sample for OCT imaging. Two water immersion objectives of 0.5N.A were used to balance the dispersion and a transverse resolution of 0.7μm was obtained. With a fast machine-coding algorithm, system sensitivity of more than 80dB and imaging rate as high as 18frame/s with 500x500 pixels per frame could be achieved. Mouse embryos were imaged in vivo with full-field OCT at different depth for the developmental study. Useful information for pre-implementation genetic diagnosis (PGD) was obtained by image analysis and segmentation. As far as known, for the fist time, 3D images of mammalian embryos were obtained with full-field OCT without the need of dye labeling.

Paper Details

Date Published: 1 May 2007
PDF: 6 pages
Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65341U (1 May 2007); doi: 10.1117/12.741591
Show Author Affiliations
Rui Wang, Tsinghua Univ. (China)
Ping Xue, Tsinghua Univ. (China)
Wenjia Bai, Tsinghua Univ. (China)
Dieyan Chen, Tsinghua Univ. (China)


Published in SPIE Proceedings Vol. 6534:
Fifth International Conference on Photonics and Imaging in Biology and Medicine

© SPIE. Terms of Use
Back to Top