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Proceedings Paper

Dynamic activation of H-Ras induced by low power laser irradiation in living cells
Author(s): Xuejuan Gao; Da Xing; Yihui Pei; Fang Wang
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Paper Abstract

Low-power laser irradiation (LPLI) has been shown to promote cell proliferation in various cell types, yet the mechanism of which has not been fully clarified. Studying the signaling pathways involved in the laser irradiation is important for understanding these processes. The Ras/Raf/MEK/ERK (extracellular-signal-regulated kinase) signaling pathway is a network that governs proliferation, differentiation and cell survival. Recent studies suggest that Ras/Raf signaling pathway is involved in the LPLI-induced cell proliferation, but the dynamic activation of Ras in living cells induced by LPLI has not been reported. In present study, to monitor the dynamic activation of H-Ras after LPLI treatment in living cells in real time, Raichu-Ras reporter was utilized, which was constructed based on fluorescence resonance energy transfer (FRET) technique. Our results show that the dynamic activation of H-Ras at the cell edges of the plasma membrane is monitored during cell proliferation induced by LPLI (0.8 J/cm2) in serum-starved COS-7 cells expressing Raichu-Ras reporter using FRET imaging on laser scanning confocal microscope, and that LPLI causes the redistribution of H-Ras from the cytoplasm to plasma membrane. The same results are observed in EGF treated COS-7 cells. Taken together, LPLI induces the COS-7 cells proliferation by activated Ras.

Paper Details

Date Published: 1 May 2007
PDF: 8 pages
Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65341D (1 May 2007); doi: 10.1117/12.741572
Show Author Affiliations
Xuejuan Gao, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Yihui Pei, South China Normal Univ. (China)
Fang Wang, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 6534:
Fifth International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin; Min Gu, Editor(s)

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