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Proceedings Paper

Optical imaging of TNF-a induced apoptosis pathway in living PC12 cells
Author(s): Lan Zhang; Da Xing; Miaojuan Chen
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Paper Abstract

Tumor necrosis factor-α (TNF-α) elicits a wide range of biological responses, including neuronal apoptosis and neuroprotection, and this functional pleiotropy is essentially determined by the individual molecular orchestration. Two main pathways lead to apoptosis - the 'extrinsic' or death receptor-initiated pathway, and the 'intrinsic' or mitochondrial pathway. In this study we firstly examine the signaling pathways involved in TNF-α induced apoptosis in living PC12 cells by optical imaging. Our results show that the cleavage of BID has been monitored in real time using fluorescence resonance energy transfer (FRET) technique after PC12 cells treated with TNF-α. Then we observe BAX can't translocation to mitochondria during PC12 cells apoptosis induced by TNF-α, and that there is no any evidence of cytochrome C release into cytosol during cell apoptosis. Our data support that TNF-α mediated PC12 cells apoptosis is extrinsic apoptotic pathway which independent of mitochondria.

Paper Details

Date Published: 1 May 2007
PDF: 9 pages
Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65341C (1 May 2007); doi: 10.1117/12.741570
Show Author Affiliations
Lan Zhang, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Miaojuan Chen, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 6534:
Fifth International Conference on Photonics and Imaging in Biology and Medicine

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