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Proceedings Paper

Sub-cell FRET imaging for determination of signaling pathway of cell apoptosis during tumor therapy
Author(s): Lei Liu; Da Xing; Wei R. Chen
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Paper Abstract

The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial and temporal resolution, distance range, and sensitivity and a broader range of biological applications. In recent years, a large number of studies have been conducted aiming to understand the process of apoptosis during tumor therapy on a molecular basis. Here, we utilized a recombinant FRET Bid probe to determine the kinetics of Bid cleavage during Cisplatin-induced apoptosis in ASTC-a-1 cells. Cells treated with Cisplatin (20 μM) showed a cleavage of the Bid-FRET probe, occurring at about 4-5 h after onset of the Cisplatin exposure, and lasted about 1.5 h. Our data clearly showed the kinetics of Bid cleavage during Cisplatin-induced apoptosis. We also used FRET technique to measure the dynamics of caspase-3 activation during apoptosis induced by high fluence low-power laser irradiation (LPLI). The data showed that we can detect caspase-3 activation sensitively and effectively, by using the FRET probe SCAT3.

Paper Details

Date Published: 1 May 2007
PDF: 10 pages
Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 653402 (1 May 2007); doi: 10.1117/12.741092
Show Author Affiliations
Lei Liu, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Wei R. Chen, South China Normal Univ. (China)
Univ. of Central Oklahoma (United States)

Published in SPIE Proceedings Vol. 6534:
Fifth International Conference on Photonics and Imaging in Biology and Medicine

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