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Proceedings Paper

Ultra-violet laser microbeam and optical trapping for cell micromanipulation
Author(s): D. Kotsifaki; M. Makropoulou; A. A. Serafetinides
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Paper Abstract

In recent years, lasers for optical trapping and micromanipulation of microscopic particles or cells and subcellular structures, both in vivo and in vitro, have gained remarkable interest in biomedical research and applications. On the other hand, a highly focused pulsed laser allows ablation and microdissection of biological material with high spatial resolution. In 1989 the microbeam field and the optical trapping field were merged by the first combination of laser microbeams and optical tweezers. Several laser sources are employed for the combination of a laser scalpel with an optical trapping device, under microscopic control. For example a pulsed laser microbeam that emits in the ultraviolet or the visible range of the electromagnetic spectrum can be used to form submicrometer cuts in biological material or to ablate parts specific cells. However, although the principles and the mechanisms of pulsed laser ablation have been well described for macroscopic interventions, the microbeam operation under microscopic guidance necessitates further investigation. In this work, we present the research and development efforts towards a pulsed UV microbeam laser system, the design and realization efforts towards a visible laser trapping system and the first results obtained on yeast cells by the combined system. The nitrogen laser microbeam setup, with special UV mirrors, lenses and a microscope in which ablation and the He-Ne optical tweezers are combined, performs the microsurgery and micromanipulation. This Uv laser based setup provides good spatial resolution for microdissection. The beam quality delivered by the laser is of great importance in microscopy controlled ablating operations and therefore was extensively studied.

Paper Details

Date Published: 26 April 2007
PDF: 9 pages
Proc. SPIE 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII, 65351O (26 April 2007); doi: 10.1117/12.741079
Show Author Affiliations
D. Kotsifaki, National Technical Univ. of Athens (Greece)
M. Makropoulou, National Technical Univ. of Athens (Greece)
A. A. Serafetinides, National Technical Univ. of Athens (Greece)

Published in SPIE Proceedings Vol. 6535:
Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII
Valery V. Tuchin, Editor(s)

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