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Proceedings Paper

A high-resolution optical imaging system for obtaining the serial transverse section images of biologic tissue
Author(s): Li Wu; Bin Zhang; Ping Wu; Qian Liu; Hui Gong
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Paper Abstract

A high-resolution optical imaging system was designed and developed to obtain the serial transverse section images of the biologic tissue, such as the mouse brain, in which new knife-edge imaging technology, high-speed and high-sensitive line-scan CCD and linear air bearing stages were adopted and incorporated with an OLYMPUS microscope. The section images on the tip of the knife-edge were synchronously captured by the reflection imaging in the microscope while cutting the biologic tissue. The biologic tissue can be sectioned at interval of 250 nm with the same resolution of the transverse section images obtained in x and y plane. And the cutting job can be automatically finished based on the control program wrote specially in advance, so we save the mass labor of the registration of the vast images data. In addition, by using this system a larger sample can be cut than conventional ultramicrotome so as to avoid the loss of the tissue structure information because of splitting the tissue sample to meet the size request of the ultramicrotome.

Paper Details

Date Published: 26 April 2007
PDF: 6 pages
Proc. SPIE 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII, 65350Z (26 April 2007); doi: 10.1117/12.740969
Show Author Affiliations
Li Wu, Huazhong Univ. of Science and Technology (China)
Bin Zhang, Huazhong Univ. of Science and Technology (China)
Ping Wu, Huazhong Univ. of Science and Technology (China)
Qian Liu, Huazhong Univ. of Science and Technology (China)
Hui Gong, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 6535:
Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII

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