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Proceedings Paper

On the way to subcellular imaging of mechanotransduction in the developing vasculature
Author(s): Irina V. Larina; Yingxiao Wang; Shu Chien; Mary E. Lane; Mary E. Dickinson
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Paper Abstract

Endothelial cells that comprise vessels and line the heart are known to respond to mechanical forces imparted by fluid flow. It is also known that blood flow is required for vascular remodeling and that abnormal heart contractions lead to the failure of the vasculature to remodel properly. Although there is considerable evidence to indicate that flow is necessary, little is known about how mechanical signals are transduced in endothelial cells in the embryo. This project is focused on understanding the role mechanical forces play in the development of the cardiovascular system using recently generated FRET (Fluorescence Resonance Energy Transfer) reporter that can detect real-time Src-kinase activity in cells using fluorescence microscopy. Src kinase regulates integrin-cytoskeleton interactions that are essential for mechanotransduction, and its activity is upregulated in cultured endothelial cells exposed to flow. Experiments reported here were focused on testing potential feasibility of the proposed technique to sense Src changes in vivo. Successful implementation of this project will reveal previously unknown signaling events involved in the mechanism of vascular remodeling and their relation to the blood flow, thus providing a unique tool for in vivo sub-cellular imaging of mechanotransduction in the vasculature and other organs.

Paper Details

Date Published: 7 May 2007
PDF: 6 pages
Proc. SPIE 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII, 653504 (7 May 2007); doi: 10.1117/12.740602
Show Author Affiliations
Irina V. Larina, Baylor College of Medicine (United States)
Yingxiao Wang, Univ. of California, San Diego (United States)
Univ. of Illinois at Urbana-Champaign (United States)
Shu Chien, Univ. of California, San Diego (United States)
Mary E. Lane, Rice Univ. (United States)
Mary E. Dickinson, Baylor College of Medicine (United States)


Published in SPIE Proceedings Vol. 6535:
Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII

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