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Proceedings Paper

Analysis of cellular structure and dynamics with digital holographic microscopy
Author(s): P. Marquet; B. Rappaz; F. Charrière; Y. Emery; C. Depeursinge; P. Magistretti
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Paper Abstract

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically, the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We discuss some approaches allowing to directly obtain separate measurements of the thickness and the refractive index (RI) of a given living cell from the phase signal.

Paper Details

Date Published: 13 July 2007
PDF: 5 pages
Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66330F (13 July 2007); doi: 10.1117/12.738579
Show Author Affiliations
P. Marquet, Ctr. de Neurosciences Psychiatriques, CHUV (Switzerland)
B. Rappaz, Brain Mind Institute, EPFL (Switzerland)
F. Charrière, Imaging and Applied Optics Institute, EPFL (Switzerland)
Y. Emery, Lyncée Tec SA, PSE-A (Switzerland)
C. Depeursinge, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
P. Magistretti, Ctr. de Neurosciences Psychiatriques, CHUV (Switzerland)
Brain Mind Institute, EPFL (Switzerland)

Published in SPIE Proceedings Vol. 6633:
Biophotonics 2007: Optics in Life Science
Jürgen Popp; Gert von Bally, Editor(s)

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