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Proceedings Paper

Increased throughput single molecule detection of DNA
Author(s): Rajan Gurjar; Madhavi Seetamraju; Noah Kolodziejski; Richard Myers; Christopher Staples; James Christian; Michael R Squillante; Gerald Entine
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Paper Abstract

In this work, we present research in using confocal optical techniques with femtolitre focal volumes and obtain very high signal-to-noise and signal-to-background ratios for single molecule detection (SMD). We were able to achieve improved signal strength by using highly fluorescent quantum dots and nanopatterned substrates to obtain plasmon induced resonant fluorescence enhancement. A method to simultaneously using multiple excitation spots without the use of confocal apertures and an array of single photon sensitive Geiger mode avalanche photodiodes was used to increase the throughput of the detection system. Using this highly sensitive SMD system, we detect small quantities of synthetic DNA through hybridization eliminating the need of polymerase chain reaction.

Paper Details

Date Published: 12 October 2007
PDF: 12 pages
Proc. SPIE 6770, Fiber Optic Sensors and Applications V, 677010 (12 October 2007); doi: 10.1117/12.737323
Show Author Affiliations
Rajan Gurjar, Radiation Monitoring Devices, Inc. (United States)
Madhavi Seetamraju, Radiation Monitoring Devices, Inc. (United States)
Noah Kolodziejski, Radiation Monitoring Devices, Inc. (United States)
Richard Myers, Radiation Monitoring Devices, Inc. (United States)
Christopher Staples, Radiation Monitoring Devices, Inc. (United States)
James Christian, Radiation Monitoring Devices, Inc. (United States)
Michael R Squillante, Radiation Monitoring Devices, Inc. (United States)
Gerald Entine, Radiation Monitoring Devices, Inc. (United States)


Published in SPIE Proceedings Vol. 6770:
Fiber Optic Sensors and Applications V
Eric Udd, Editor(s)

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