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Proceedings Paper

Bio-functional Au/Si nanorods for pathogen detection
Author(s): Bosoon Park; Junxue Fu; Yiping Zhao; Gregory R. Siragusa; Yong-Jin Cho; Kurt C. Lawrence; William R. Windham
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Paper Abstract

Nanotechnology applications for food safety and biosecurity, especially development of nanoscale sensors for foodborne pathogen measurement are emerging. A novel bio-functional nanosensor for Salmonella detection was developed using hetero-nanorods. The silica nanorods were fabricated by glancing angle deposition method and the gold was sputtered onto the silica nanorods. Alexa488-succinimide dye was immobilized onto the annealed Si nanorods via the attachment between dye ester and primary amine group supplied by the 3-Aminopropyltriethoxysilane. The anti-Salmonella was conjugated to gold via Dithiobis[succinimidylpropionate] self-assembly monolayer. Due to the high aspect ratio nature of the Si nanorods, hundreds or thousands of dye molecules attached to the Si nanorods produced enhanced fluorescence signal. These biologically functionalized nanorods can be used to detect Salmonella with fluorescent microscopic imaging. This new nanoscale biosensor will be able to detect other foodborne pathogenic bacteria for food safety and security applications.

Paper Details

Date Published: 25 September 2007
PDF: 9 pages
Proc. SPIE 6769, Nanosensing: Materials, Devices, and Systems III, 67690O (25 September 2007); doi: 10.1117/12.736486
Show Author Affiliations
Bosoon Park, USDA Agricultural Research Service (United States)
Junxue Fu, Univ. of Georgia (United States)
Yiping Zhao, Univ. of Georgia (United States)
Gregory R. Siragusa, USDA Agricultural Research Service (United States)
Yong-Jin Cho, Korea Food Research Institute (South Korea)
Kurt C. Lawrence, USDA Agricultural Research Service (United States)
William R. Windham, USDA Agricultural Research Service (United States)

Published in SPIE Proceedings Vol. 6769:
Nanosensing: Materials, Devices, and Systems III
M. Saif Islam; Achyut K. Dutta, Editor(s)

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