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Proceedings Paper

Multi-dimensional time-correlated single-photon counting
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Paper Abstract

Time-correlated single photon counting (TCSPC) is based on the detection of single photons of a periodic light signal, measurement of the detection time of the photons, and the build-up of the photon distribution versus the time in the signal period. TCSPC achieves a near ideal counting efficiency and transit-time-spread-limited time resolution for a given detector. We present an advanced TCSPC technique featuring multi-dimensional photon acquisition and a count rate close to the capability of currently available detectors. The technique is able to acquire photon distributions versus wavelength, spatial coordinates, and the time on the ps scale, and to record fast changes in the fluorescence lifetime and fluorescence intensity of a sample. Biomedical applications of advanced TCSPC techniques include time-domain optical tomography, recording of transient phenomena in biological systems, spectrally resolved fluorescence lifetime imaging, FRET experiments in living cells, and the investigation of dye-protein complexes by fluorescence correlation spectroscopy. We demonstrate the potential of the technique for selected applications.

Paper Details

Date Published: 11 October 2007
PDF: 10 pages
Proc. SPIE 6771, Advanced Photon Counting Techniques II, 677102 (11 October 2007); doi: 10.1117/12.735546
Show Author Affiliations
Wolfgang Becker, Becker and Hickl GmbH (Germany)
Axel Bergmann, Becker and Hickl GmbH (Germany)


Published in SPIE Proceedings Vol. 6771:
Advanced Photon Counting Techniques II
Wolfgang Becker, Editor(s)

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