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Proceedings Paper

Oxytetracycline analysis in honey using a specific portable analyzer
Author(s): Guoying Chen; Daniel Schwartz; S. Braden; Alberto Nunez
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Paper Abstract

Oxytetracycline (OTC) residue in honey is detected using a portable analyzer designed to specifically target tetracycline (TC) drugs based on europium-sensitized luminescence (ESL). A 385 nm light emitting diode (LED) is used as the excitation source and a photomultiplier tube as the light detector. OTC is extracted from honey and cleaned up by solid phase extraction (SPE) using Strata X-WC weak cation exchange cartridges. To the eluate Eu(III) is added to form a Eu-TC chelate at pH 8.5. Efficient intrachelate energy transfer allows sensitive OTC detection at λex=385 nm and λem=610 nm. After a 25-µs time delay, the ESL signal is integrated over a 25-1000 µs interval. The signal intensity reveals a linear relationship (R2=0.972) to OTC concentrations in the 10-200 ng/g range. The limit-of-detection is 6.7 ng/g with an average 5.8% relative standard deviation. The background signal corresponds to ~10 ppb. This instrumentation and method combination enables field analysis that is especially useful for beekeeping industry.

Paper Details

Date Published: 22 October 2007
PDF: 7 pages
Proc. SPIE 6761, Optics for Natural Resources, Agriculture, and Foods II, 67610K (22 October 2007); doi: 10.1117/12.735029
Show Author Affiliations
Guoying Chen, USDA Agricultural Research Service (United States)
Daniel Schwartz, USDA, Agricultural Research Service (United States)
S. Braden, USDA Agricultural Research Service (United States)
Alberto Nunez, USDA Agricultural Research Service (United States)

Published in SPIE Proceedings Vol. 6761:
Optics for Natural Resources, Agriculture, and Foods II
Yud-Ren Chen; George E. Meyer; Shu-I Tu, Editor(s)

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