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Proceedings Paper

Encoding arbitrary grey-level optical landscapes for trapping and manipulation using GPC
Author(s): Carlo Amadeo Alonzo; Peter John Rodrigo; Darwin Palima; Jesper Glückstad
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Paper Abstract

With the aid of phase-only spatial light modulators (SLM), generalized phase contrast (GPC) has been applied with great success to the projection of binary light patterns through arbitrary-NA microscope objectives for real-time threedimensional manipulation of microscopic particles. Here, we review the analysis of the GPC method with emphasis on efficiently producing speckle-free two-dimensional grey-level light patterns. Numerical simulations are applied to construct 8-bit grey-level optical potential landscapes with high fidelity and optical throughput via the GPC method. Three types of patterns were constructed: geometric block patterns, multi-level optical trap arrays, and optical obstacle arrays. Non-periodic patterns were accurately projected with an average of 80% diffraction efficiency. Periodic patterns yielded even higher diffraction efficiencies, averaging 94%, by the utilization of large-aperture phase contrast filters.

Paper Details

Date Published: 5 September 2007
PDF: 9 pages
Proc. SPIE 6644, Optical Trapping and Optical Micromanipulation IV, 66440X (5 September 2007); doi: 10.1117/12.733956
Show Author Affiliations
Carlo Amadeo Alonzo, Technical Univ. of Denmark (Denmark)
Peter John Rodrigo, Technical Univ. of Denmark (Denmark)
Darwin Palima, Technical Univ. of Denmark (Denmark)
Jesper Glückstad, Technical Univ. of Denmark (Denmark)

Published in SPIE Proceedings Vol. 6644:
Optical Trapping and Optical Micromanipulation IV
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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