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Proceedings Paper

Spectrally and time-resolved study of NAD(P)H autofluorescence in cardiac myocytes from human biopsies
Author(s): Y. Cheng; D. Chorvat; N. Poirier; J. Miró; N. Dahdah; A. Chorvatova
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Paper Abstract

Rejection of transplanted hearts remains an important reason for death of transplanted children. Finding diagnostic tools for its detection can therefore improve the prognosis in this population of patients. Endomyocardial biopsy (EMB) by cardiac catheterization is currently accepted as the "gold standard" for the diagnosis of rejection. Here, we investigate new approach to monitor mitochondrial metabolic state of cardiac cells using spectrally-resolved autofluorescence lifetime detection of nicotinamide adenine dinucleotide (phosphate), or NAD(P)H, the principal electron donor in mitochondrial oxidative energy metabolism responsible for vital ATP supply of cardiomyocytes. NAD(P)H autofluorescence is long used for non-invasive fluorescent probing the metabolic state of the heart. In this contribution we report dynamic characteristics of NAD(P)H fluorescence decays in living human cardiomyocytes from EMB, following excitation by UV-pulsed laser diode and detection by spectrally-resolved time-correlated single photon counting. At least a 3-exponential decay model, with 0.5-0.7 ns, 1.9-2.4 ns and 9.0-15.0 ns lifetimes, is necessary to describe cardiomyocyte autofluorescence in human cells. When gathered data were compared to those recorded under same conditions in rats, autofluorescence in human hearts was found significantly lower in comparison to rat ones. Rotenone, the inhibitor of the Complex I of the respiratory chain, increased the fluorescence in human cardiac cells, making them more comparable to experimental rat model. These results suggest that human cardiac cells are more metabolically active than the rat ones in the same conditions. Presented work proposes a new tool for evaluation of oxidative metabolism changes in transplanted hearts.

Paper Details

Date Published: 15 October 2007
PDF: 13 pages
Proc. SPIE 6771, Advanced Photon Counting Techniques II, 677104 (15 October 2007); doi: 10.1117/12.732876
Show Author Affiliations
Y. Cheng, CHU Sainte-Justine (Canada)
D. Chorvat, International Laser Ctr. (Slovak Republic)
N. Poirier, CHU Sainte-Justine (Canada)
Univ. of Montréal (Canada)
J. Miró, CHU Sainte-Justine (Canada)
Univ. of Montréal (Canada)
N. Dahdah, CHU Sainte-Justine (Canada)
Univ. of Montréal (Canada)
A. Chorvatova, CHU Sainte-Justine (Canada)
Univ. of Montréal (Canada)


Published in SPIE Proceedings Vol. 6771:
Advanced Photon Counting Techniques II
Wolfgang Becker, Editor(s)

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