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Proceedings Paper

Spectrally resolved laser-induced fluorescence for bioaerosols standoff detection
Author(s): Sylvie Buteau; Laurie Stadnyk; Susan Rowsell; Jean-Robert Simard; Jim Ho; Bernard Déry; John McFee
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Paper Abstract

An efficient standoff biological warfare detection capability could become an important asset for both defence and security communities based on the increasing biological threat and the limits of the presently existing protection systems. Defence R&D Canada (DRDC) has developed, by the end of the 90s, a standoff bioaerosol sensor prototype based on intensified range-gated spectrometric detection of Laser Induced Fluorescence (LIF). This LIDAR system named SINBAHD monitors the spectrally resolved LIF originating from inelastic interactions with bioaerosols present in atmospheric cells customizable in size and in range. SINBAHD has demonstrated the capability of near real-time detection and classification of bioaerosolized threats at multi-kilometre ranges. In spring 2005, DRDC has initiated the BioSense demonstration project, which combines the SINBAHD technology with a geo-referenced Near InfraRed (NIR) LIDAR cloud mapper. SINBAHD is now being used to acquire more signatures to add in the spectral library and also to optimize and test the new BioSense algorithm strategy. In September 2006, SINBAHD has participated in a two-week trial held at DRDC-Suffield where different open-air wet releases of live and killed bioagent simulants, growth media and obscurants were performed. An autoclave killing procedure was performed on two biological materials (Bacillus subtilis var globigii or BG, and Bacillus thuringiensis or Bt) before being aerosolized, disseminated and spectrally characterized with SINBAHD. The obtained results showed no significant impact of this killing process on their normalised spectral signature in comparison with their live counterparts. Correlation between the detection signals from SINBAHD, an array of slit samplers and a FLuorescent Aerosol Particle Sensor (C-FLAPS) was obtained and SINBAHD's sensitivity could then be estimated. At the 2006 trial, a detection limit of a few tens of Agent Containing Particles per Liter of Air (ACPLA) was obtained for a 15-m thick cloud of live BG located at a range of 400 m.

Paper Details

Date Published: 25 September 2007
PDF: 10 pages
Proc. SPIE 6756, Chemical and Biological Sensors for Industrial and Environmental Monitoring III, 675608 (25 September 2007); doi: 10.1117/12.732389
Show Author Affiliations
Sylvie Buteau, Defence Research and Development Canada (Canada)
Laurie Stadnyk, Defence Research and Development Canada (Canada)
Susan Rowsell, Defence Research and Development Canada (Canada)
Jean-Robert Simard, Defence Research and Development Canada (Canada)
Jim Ho, Defence Research and Development Canada (Canada)
Bernard Déry, Defence Research and Development Canada (Canada)
John McFee, Defence Research and Development Canada (Canada)


Published in SPIE Proceedings Vol. 6756:
Chemical and Biological Sensors for Industrial and Environmental Monitoring III
Kenneth J. Ewing; James B. Gillespie; Pamela M. Chu; William J. Marinelli, Editor(s)

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