Share Email Print
cover

Proceedings Paper

High-resolution light microscopy of nanoforms
Author(s): Vitaly Vodyanoy; Oleg Pustovyy; Arnold Vainrub
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

We developed a high resolution light imaging system. Diffraction gratings with 100 nm width lines as well as less than 100 nm size features of different-shaped objects are clearly visible on a calibrated microscope test slide (Vainrub et al., Optics Letters, 2006, 31, 2855). The two-point resolution increase results from a known narrowing of the central diffraction peak for the annular aperture. Better visibility and advanced contrast of the smallest features in the image are due to enhancement of high spatial frequencies in the optical transfer function. The imaging system is portable, low energy, and battery operated. It has been adapted to use in both transmitting and reflecting light. It is particularly applicable for motile nanoform systems where structure and functions can be depicted in real time. We have isolated micrometer and submicrometer particles, termed proteons, from human and animal blood. Proteons form by reversible seeded aggregation of proteins around proteon nucleating centers (PNCs). PNCs are comprised of 1-2nm metallic nanoclusters containing 40-300 atoms. Proteons are capable of spontaneous assembling into higher nanoform systems assuming structure of complicated topology. The arrangement of complex proteon system mimics the structure of a small biological cell. It has structures that imitate membrane and nucleolus or nuclei. Some of these nanoforms are motile. They interact and divide. Complex nanoform systems can spontaneously reduce to simple proteons. The physical properties of these nanoforms could shed some light on the properties of early life forms or forms at extreme conditions.

Paper Details

Date Published: 1 October 2007
PDF: 12 pages
Proc. SPIE 6694, Instruments, Methods, and Missions for Astrobiology X, 669413 (1 October 2007); doi: 10.1117/12.731462
Show Author Affiliations
Vitaly Vodyanoy, Auburn Univ. (United States)
Oleg Pustovyy, Auburn Univ. (United States)
Arnold Vainrub, Auburn Univ. (United States)


Published in SPIE Proceedings Vol. 6694:
Instruments, Methods, and Missions for Astrobiology X
Richard B. Hoover; Gilbert V. Levin; Alexei Y. Rozanov; Paul C. W. Davies, Editor(s)

© SPIE. Terms of Use
Back to Top