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Proceedings Paper

2D and 3D printing of biomolecules employing femtosecond lasers
Author(s): Maria Farsari; V. Dinca; M. Dinescu; T. S. Drakakis; C. Fotakis
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Paper Abstract

We demonstrate two- and three-dimensional patterning of biological molecules. For the two-dimensional patterning we employ Laser-Induced Forward Transfer of materials in solution. For the three-dimensional patterning we employ femtosecond-laser induced three-photon polymerization, a technique which enables the construction of arbitrary 2D and 3D structures of submicron resolution. Biotin is subsequently attached to the 3D structures via UV-activated crosslinking. The integrity of the photolytically immobilized biotin is confirmed by detecting the binding of fluorescently labeled avidin via fluorescence microscopy.

Paper Details

Date Published: 25 April 2007
PDF: 7 pages
Proc. SPIE 6606, Advanced Laser Technologies 2006, 66061F (25 April 2007); doi: 10.1117/12.730199
Show Author Affiliations
Maria Farsari, Foundation for Research and Technology Hellas (Greece)
V. Dinca, Foundation for Research and Technology Hellas (Greece)
National Institute for Laser, Plasma and Radiation Physics (Romania)
M. Dinescu, National Institute for Laser, Plasma and Radiation Physics (Romania)
T. S. Drakakis, Foundation for Research and Technology Hellas (Greece)
C. Fotakis, Foundation for Research and Technology Hellas (Greece)


Published in SPIE Proceedings Vol. 6606:
Advanced Laser Technologies 2006

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