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Proceedings Paper

Two- and one-photon color confocal screening microscope
Author(s): Christian Seebacher; Joachim Walter; Rainer Uhl
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Paper Abstract

The goal was to develop an upright microscope platform for the screening of slides employing one- and two-photon laser scanning techniques. A highly compact, vibration damping unit was created, which combines novel concepts for moving a slide in three dimensions, keeping it focused while doing so, scanning a laser-focus over the sample using novel galvanometer-control concepts, combining and separating excitation and emission beam and spectrally dispersing the emitted light by a linearized prism-spectrograph. Spectral detection is achieved by turning a 128 x 128 back-thinned EMCCD detector in a continuously reading spectral point-detector. To make the unit even ore versatile, it can be turned into a conventional wide field fluorescence microscope, enabling rapid routine observation to select regions of the sample for a subsequent, more detailed confocal analysis.

Paper Details

Date Published: 12 July 2007
PDF: 11 pages
Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 66300F (12 July 2007); doi: 10.1117/12.729553
Show Author Affiliations
Christian Seebacher, BioImaging Zentrum, LMU Munich (Germany)
Joachim Walter, TILL I.D. GmbH (Germany)
Rainer Uhl, BioImaging Zentrum, LMU Munich (Germany)
TILL I.D. GmbH (Germany)
TILL Photonics GmbH (Germany)

Published in SPIE Proceedings Vol. 6630:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging III
Tony Wilson; Ammasi Periasamy, Editor(s)

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