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Proceedings Paper

Rigid and high NA multiphoton fluorescence GRIN-endoscopes
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Paper Abstract

Multiphoton autofluorescence imaging offers minimal-invasive examination of cells without the need of staining and complicated confocal detection systems. Therefore, it is especially interesting for non-invasive clinical diagnostics. To extend this sophisticated technique from superficial regions to deep lying cell layers, internal body parts and specimens difficult of access, the bulky optics need to be reduced in diameter. This is done by tiny GRIN-optics, based on a radial gradient in the reflective index. Of especial interest for multi-photon applications is the newly developed GRIN-lens assembly with increased numerical aperture. High resolution images of plant tissue, hair and cells show the improved image quality,compared to classical GRIN-lenses. The rigid GRIN-endoscopes are already applied in wound healing studies. Here, the GRIN-lenses with diameters smaller than 3 mm enter small skin depressions. They reproduce the focus of a conventional laser scanning tomograph tens of mm apart in the specimen under study. We present first clinical measurements of elastin and SHG of collagen of in-vivo human skin of venous ulcers (ulcer curis).

Paper Details

Date Published: 18 July 2007
PDF: 7 pages
Proc. SPIE 6631, Novel Optical Instrumentation for Biomedical Applications III, 66310Q (18 July 2007); doi: 10.1117/12.728503
Show Author Affiliations
Selma Schenkl, Fraunhofer Institute of Biomedical Technology (Germany)
Saarland Univ. (Germany)
Alexander Ehlers, Fraunhofer Institute of Biomedical Technology (Germany)
Saarland Univ. (Germany)
Ronan Le Harzic, Fraunhofer Institute of Biomedical Technology (Germany)
Jenlab GmbH (Germany)
Martin Stark, Fraunhofer Institute of Biomedical Technology (Germany)
Iris Riemann, Fraunhofer Institute of Biomedical Technology (Germany)
Bernhard Messerschmidt, Grintech GmbH (Germany)
Martin Kaatz, Friedrich-Schiller-Univ. Jena (Germany)
Karsten König, Fraunhofer Institute of Biomedical Technology (Germany)
Saarland Univ. (Germany)


Published in SPIE Proceedings Vol. 6631:
Novel Optical Instrumentation for Biomedical Applications III
Christian D. Depeursinge, Editor(s)

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