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Proceedings Paper

Fluorescence lifetime imaging through turbid media reconstructed in the Fourier domain using time-gated imaging data
Author(s): Vadim Y. Soloviev; Khadija B. Tahir; James McGinty; Dan S. Elson; Mark Neil; Alessandro Sardini; Joseph Hajnal; Simon R. Arridge; Paul M. W. French
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Paper Abstract

We report a novel technique to reconstruct fluorescence lifetime distributions in turbid media by using Fourier domain reconstruction of time gated imaging data. The time gating provides sufficient temporal resolution to determine short fluorescence lifetimes while the use of the Fourier transform, which is essential for the time de-convolution of the system of the integral equations employed in the reconstruction, permits a relatively rapid reconstruction of 3-D tomographic data. This approach has been applied experimentally to reconstruct fluorescent lifetime distributions corresponding to phantoms with wells filled with fluorescent dyes embedded inside highly scattering slabs. In practice, the scattering medium can itself be fluorescent and we also suggest a simple iterative technique to account for background autofluorescence, which we have also tested experimentally.

Paper Details

Date Published: 12 July 2007
PDF: 9 pages
Proc. SPIE 6629, Diffuse Optical Imaging of Tissue, 66291V (12 July 2007); doi: 10.1117/12.728481
Show Author Affiliations
Vadim Y. Soloviev, Univ. College London (United Kingdom)
Khadija B. Tahir, Imperial College London (United Kingdom)
James McGinty, Imperial College London (United Kingdom)
Dan S. Elson, Imperial College London (United Kingdom)
Mark Neil, Imperial College London (United Kingdom)
Alessandro Sardini, Imperial College Faculty of Medicine (United Kingdom)
Joseph Hajnal, Imperial College Faculty of Medicine (United Kingdom)
Simon R. Arridge, Univ. College London (United Kingdom)
Paul M. W. French, Imperial College London (United Kingdom)

Published in SPIE Proceedings Vol. 6629:
Diffuse Optical Imaging of Tissue
Brian W. Pogue; Rinaldo Cubeddu, Editor(s)

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