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Proceedings Paper

Applications of rapid time-gated hyperspectral FLIM: live cell imaging of membrane order and 6-D microscopy
Author(s): H. B Manning; D. M Owen; E. Auksorius; P. A. A. de Beule; S. Oddos; C. B Talbot; C. Dunsby; I. Munro; A. I Magee; M. A. A. Neil; P. M. W. French
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Paper Abstract

We describe the characterisation of a hyperspectral fluorescence lifetime imaging microscope that exploits high-speed time-gated imaging technology and a tunable continuum source for 6-D fluorescence imaging. This line-scanning confocal microscope can record the full spectral-temporal (i.e. excitation-emission-lifetime) fluorescence matrix at each pixel in a three dimensional (x-y-z) sample. This instrument has been applied to biological samples including model membranes and live cells labelled with the phase-sensitive membrane dye di-4-ANEPPDHQ, for which significant variation of lifetime with emission wavelength is observed.

Paper Details

Date Published: 13 July 2007
PDF: 12 pages
Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 663019 (13 July 2007); doi: 10.1117/12.728479
Show Author Affiliations
H. B Manning, Imperial College London (United Kingdom)
D. M Owen, Imperial College London (United Kingdom)
E. Auksorius, Imperial College London (United Kingdom)
P. A. A. de Beule, Imperial College London (United Kingdom)
S. Oddos, Imperial College London (United Kingdom)
C. B Talbot, Imperial College London (United Kingdom)
C. Dunsby, Imperial College London (United Kingdom)
I. Munro, Imperial College London (United Kingdom)
A. I Magee, Imperial College London (United Kingdom)
M. A. A. Neil, Imperial College London (United Kingdom)
P. M. W. French, Imperial College London (United Kingdom)


Published in SPIE Proceedings Vol. 6630:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging III
Tony Wilson; Ammasi Periasamy, Editor(s)

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