Share Email Print

Proceedings Paper

Fluorescence diffuse tomography for detection of RFP-expressed tumors in small animals
Author(s): Ilya V. Turchin; Alexander P. Savitsky; Vladislav A. Kamensky; Vladimir I. Plehanov; Anna G. Orlova; Mikhail S. Kleshnin; Marina V. Shirmanova; Ilya I. Fix; Vladimir O. Popov
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Capabilities of tumor detection by different optical methods can be significantly improved by labeling of tumors with fluorescent markers. Creation of tumor cell lines transfected with fluorescent proteins provides the possibility not only to detect tumor, but also to conduct the intravital monitoring studies. Cell lines of human melanomas Mel-P, Mel-Kor and human embryonic kidney HEK-293 Phoenix were transfected with DsRed-Express and Turbo-RFP genes. Emission of RFP in the long-wave optical range permits detection of the deeply located tumors, which is essential for whole-body imaging. Only special tools for turbid media imaging, such as fluorescent diffusion tomography (FDT), enable noninvasive investigation of the internal structure of biological tissue. FDT setup for monitoring of tumor growth in small animals has been created. An animal is scanned in the transilluminative configuration by low-frequency modulated light (1 kHz) from Nd:YAG laser with second harmonic generation at the 532 nm wavelength. An optimizing algorithm for scanning of an experimantal animal is suggested. In vivo experiments were conducted immediately after the subcutaneously injection of fluorescing cells into small animals. It was shown that FDT method allows to detect the presence of fluorescent cells in small animals and can be used for monitoring of tumor growth and anticancer drug responce.

Paper Details

Date Published: 11 July 2007
PDF: 8 pages
Proc. SPIE 6626, Molecular Imaging, 66260R (11 July 2007); doi: 10.1117/12.728470
Show Author Affiliations
Ilya V. Turchin, Institute of Applied Physics (Russia)
Alexander P. Savitsky, A.N. Bach Institute of Biochemistry (Russia)
Vladislav A. Kamensky, Institute of Applied Physics (Russia)
Vladimir I. Plehanov, Institute of Applied Physics (Russia)
Anna G. Orlova, Institute of Applied Physics (Russia)
Mikhail S. Kleshnin, Institute of Applied Physics (Russia)
Marina V. Shirmanova, Institute of Applied Physics (Russia)
Ilya I. Fix, Institute of Applied Physics (Russia)
Vladimir O. Popov, A.N. Bach Institute of Biochemistry (Russia)

Published in SPIE Proceedings Vol. 6626:
Molecular Imaging
Kai Licha; Vasilis Ntziachristos, Editor(s)

© SPIE. Terms of Use
Back to Top