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Proceedings Paper

Heterodyne interference microscopy for non-invasive cell morphometry
Author(s): Maurice P. Whelan; Fereydoun Lakestani; Diana Rembges; Maria Grazia Sacco
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Paper Abstract

This paper describes two setups suitable for interference microscopy for high resolution morphological measurements on living cells in culture medium. The first system incorporated a PZT actuator in the reference path of a Mach Zehnder configuration to facilitate digital phase-stepping interferometry. The second system employed two phase-locked acoustooptic modulators to generate a temporal optical carrier to allow a heterodyne approach to phase demodulation. This setup incorporated a digital CMOS camera with full random pixel access which allowed the heterodyne approach to be implemented as a full-field method without any need for electromechanical scanning. The heterodyne approach offers benefits over the phase-stepping method in terms of measurement resolution and speed, typically offering the equivalent of nanometer resolution for cell height measurements with a bandwidth in the order of 200-300 Hz for 1000 pixels. Results for morphological measurements using both systems on red blood cells and keratinocytes are presented.

Paper Details

Date Published: 17 July 2007
PDF: 9 pages
Proc. SPIE 6631, Novel Optical Instrumentation for Biomedical Applications III, 66310E (17 July 2007); doi: 10.1117/12.728449
Show Author Affiliations
Maurice P. Whelan, European Commission DG Joint Research Ctr. (Italy)
Fereydoun Lakestani, European Commission DG Joint Research Ctr. (Italy)
Diana Rembges, European Commission DG Joint Research Ctr. (Italy)
Maria Grazia Sacco, European Commission DG Joint Research Ctr. (Italy)


Published in SPIE Proceedings Vol. 6631:
Novel Optical Instrumentation for Biomedical Applications III
Christian D. Depeursinge, Editor(s)

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