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Proceedings Paper

Development of microfluidic structures for high throughput flow cytometric characterization of blood cells
Author(s): A. Kummrow; H. Yildirim; J. Theisen; K. Brattke; C. Sprenger; M. Schmidt; J. Neukammer
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Paper Abstract

We exploited ultraprecision milling to fabricate three dimensional microfluidic structures incorporating cascaded hydrodynamic focusing. Stable operation when varying the sample flow over three orders of magnitude was demonstrated. Impedance detection or forward light scatter served to detect polystyrene microspheres and to differentiate red blood cells and blood platelets.

Paper Details

Date Published: 13 July 2007
PDF: 7 pages
Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66332C (13 July 2007); doi: 10.1117/12.728440
Show Author Affiliations
A. Kummrow, Physikalisch-Technische Bundesanstalt (Germany)
H. Yildirim, Physikalisch-Technische Bundesanstalt (Germany)
J. Theisen, Technische Univ. Berlin (Germany)
K. Brattke, Physikalisch-Technische Bundesanstalt (Germany)
C. Sprenger, Technische Univ. Berlin (Germany)
M. Schmidt, Technische Univ. Berlin (Germany)
J. Neukammer, Physikalisch-Technische Bundesanstalt (Germany)

Published in SPIE Proceedings Vol. 6633:
Biophotonics 2007: Optics in Life Science
Jürgen Popp; Gert von Bally, Editor(s)

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