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Proceedings Paper

Development of a TIRF-FLIM microscope for biomedical applications
Author(s): P. Blandin; S. Lévêque-Fort; S. Lécart; P. Zeller; Z. Lenkei; F. Druon; P. Georges
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Paper Abstract

Total internal reflection fluorescence microscopy (TIRFM) is a powerful optical technique to observe fluorescence close to surfaces. Associated with fluorescence lifetime imaging, TIRFM enables to measure contrasts independent of fluorophores concentration and reveal intracellular activity with subwavelength axial resolution. We developed an original setup which allows, thanks to a wide-field time resolved detection, to measure nanoseconds fluorescence lifetimes of membrane receptors in order to apprehend their signalisation pathway.

Paper Details

Date Published: 8 August 2007
PDF: 9 pages
Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 66300B (8 August 2007); doi: 10.1117/12.728429
Show Author Affiliations
P. Blandin, Lab. de Photophysique Moléculaire et Ctr. Laser, Univ. Paris Sud (France)
Institut d’Optique Graduate School, Campus Polytechnique (France)
S. Lévêque-Fort, Lab. de Photophysique Moléculaire et Ctr. Laser, Univ. Paris Sud (France)
S. Lécart, Lab. de Photophysique Moléculaire et Ctr. Laser, Univ. Paris Sud (France)
P. Zeller, Lab. de Neurobiologie et Diversité Cellulaire, ESPCI (France)
Z. Lenkei, Lab. de Neurobiologie et Diversité Cellulaire, ESPCI (France)
F. Druon, Institut d’Optique Graduate School, Campus Polytechnique (France)
P. Georges, Institut d’Optique Graduate School, Campus Polytechnique (France)


Published in SPIE Proceedings Vol. 6630:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging III
Tony Wilson; Ammasi Periasamy, Editor(s)

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