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Proceedings Paper

Refractive index determination by index-mismatch-induced spherical aberration
Author(s): Ping-Jung Su; Peter T. Fwu; Vladimir Hovhannisyan; Chen-Yuan Dong
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Paper Abstract

Two-photon microscopy has been successfully applied in biological and material sciences since 1990. However, it is known that the resolution of two-photon imaging is adversely affected from the index mismatch induced spherical aberrations. Spherical aberration increases the focal volume and causes degradation of image resolution as one images deeper into the specimen. In this work, we propose to use this intrinsic artifact to measure the refractive index in specimens of uniform refractive indices. Using the intensity profiles of standard refractive liquids as our reference, we can compare the intensity profile of an unknown specimen of uniform refractive index with that of the reference to determine the refractive index of the unknown specimen.

Paper Details

Date Published: 16 July 2007
PDF: 6 pages
Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 66300C (16 July 2007); doi: 10.1117/12.728387
Show Author Affiliations
Ping-Jung Su, National Taiwan Univ. (Taiwan)
Peter T. Fwu, National Taiwan Univ. (Taiwan)
Vladimir Hovhannisyan, National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6630:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging III
Tony Wilson; Ammasi Periasamy, Editor(s)

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