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Proceedings Paper

Extended focus Fourier domain optical coherence microscopy assists developmental biology
Author(s): Martin L. Villiger; Manfred Beleut; Cathrin Brisken; Theo Lasser; Rainer A. Leitgeb
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Paper Abstract

We present a novel detection scheme for Fourier domain optical coherence microscopy (FDOCM). A Bessel-like interference pattern with a strong central lobe was created with an axicon lens. This pattern was then imaged by a telescopic system into the sample space to obtain a laterally highly confined illumination needle, extending over a long axial range. For increased efficiency, the detection occurs decoupled from the illumination, avoiding a double pass through the axicon. Nearly constant transverse resolution of ~1.5&mgr;m along a focal range of 200&mgr;m with a maximum sensitivity of 105dB was obtained. A broad bandwidth Ti:Sapphire laser allowed for an axial resolution of 3&mgr;m in air, providing the nearly isotropic resolution necessary to access the microstructure of biological tissues. Together with the speed- and sensitivity-advantage of FDOCT, this system can perform in vivo measurements in a minimally invasive way. Tomograms of the mouse mammary gland and the mouse follicle, recorded in vitro, revealed biologically relevant structural details. Images acquired with classical microscopy techniques, involving stained and fluorescent samples, validate these structures and emphasize the high contrast of the tomograms. It is comparable to the contrast achieved with classical techniques, but employing neither staining, labeling nor slicing of the samples, stressing the high potential of FDOCM for minimally invasive in vivo small animal imaging.

Paper Details

Date Published: 11 July 2007
PDF: 6 pages
Proc. SPIE 6627, Optical Coherence Tomography and Coherence Techniques III, 66271H (11 July 2007); doi: 10.1117/12.728370
Show Author Affiliations
Martin L. Villiger, École Polytechnique Fédérale de Lausanne (Switzerland)
Manfred Beleut, Institut Suisse de Recherche Expérimentale sur le Cancer (Switzerland)
Cathrin Brisken, Institut Suisse de Recherche Expérimentale sur le Cancer (Switzerland)
Theo Lasser, École Polytechnique Fédérale de Lausanne (Switzerland)
Rainer A. Leitgeb, École Polytechnique Fédérale de Lausanne (Switzerland)
Medical Univ. of Vienna (Austria)


Published in SPIE Proceedings Vol. 6627:
Optical Coherence Tomography and Coherence Techniques III
Peter E. Andersen; Zhongping Chen, Editor(s)

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