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Proceedings Paper

Fluorescence study of bovine serum albumin and Ti and Sn oxide nanoparticles interactions
Author(s): Denisio M. Togashi; Alan G. Ryder; Deirdre Mc Mahon; Peter Dunne; James McManus
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Paper Abstract

Nanochemistry offers stimulating opportunities for a wide variety of applications in the biosciences. Understanding of the interaction of nanoparticles with biomolecules such as proteins is very important as it can help better design and fabricate nanocomposites for applications in diagnostics, drug delivery, and cell monitoring. In this work, the interaction of Bovine Serum Albumin (BSA) and two types of metal oxide nanoparticles (titanium and tin) have been studied using the intrinsic fluorescence of tryptophan residue from the proteins measured by steady state and time resolved fluorescence techniques. The nanoparticles which were fabricated using a novel synthetic process have average sizes of ~2 nm (SnO2) and ~6 nm (estimated for TiO2) and have very high solubilities in a variety of solvents. The Stern-Volmer plots indicate an effective quenching process by TiO2 nanoparticles whereas SnO2 nanoparticles have a lower quenching efficiency for BSA fluorescence. Static quenching is the major contribution in the overall process which may indicate a high degree of association between protein and nanoparticles. The difference in BSA fluorescence quenching efficiency between the two types of nanoparticles can be explained by the non-covalent interaction differences and the thermal stability of protein-nanoparticle associated species for both materials.

Paper Details

Date Published: 11 July 2007
PDF: 11 pages
Proc. SPIE 6628, Diagnostic Optical Spectroscopy in Biomedicine IV, 66281K (11 July 2007); doi: 10.1117/12.728354
Show Author Affiliations
Denisio M. Togashi, National Univ. of Ireland, Galway (Ireland)
Alan G. Ryder, National Univ. of Ireland, Galway (Ireland)
Deirdre Mc Mahon, National Univ. of Ireland, Galway (Ireland)
Peter Dunne, National Univ. of Ireland, Galway (Ireland)
James McManus, National Univ. of Ireland, Galway (Ireland)

Published in SPIE Proceedings Vol. 6628:
Diagnostic Optical Spectroscopy in Biomedicine IV
Dietrich Schweitzer; Maryann Fitzmaurice, Editor(s)

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