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Proceedings Paper

Dynamics and detection of laser induced microbubbles in the retinal pigment epithelium (RPE)
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Paper Abstract

Selective Retina Treatment (SRT) is a new method to treat eye diseases associated with disorders of the RPE. Selective RPE cell damage is achieved by applying a train of 1.7 μs laser pulses at 527 nm. The treatment of retinal diseases as e.g. diabetic maculopathy (DMP), is currently investigated within clinical studies, however 200 ns pulse durations are under investigation. Transient micro bubbles in the retinal pigment epithelium (RPE) are expected to be the origin of cell damage due to irradiation with laser pulses shorter than 50 μs. The bubbles emerge at the strongly absorbing RPE melanosomes. Cell membrane disruption caused by the transient associated volume increase is expected to be the origin of the angiographically observed RPE leakage. We investigate micro bubble formation and dynamics in porcine RPE using pulse durations of 150 ns. A laser interferometry system at 830 nm with the aim of an online dosimetry control for SRT was developed. Bubble formation was detected interferometrically and by fast flash photography. A correlation to cell damage observed with a vitality stain is found. A bubble detection algorithm is presented.

Paper Details

Date Published: 13 July 2007
PDF: 11 pages
Proc. SPIE 6632, Therapeutic Laser Applications and Laser-Tissue Interactions III, 66321C (13 July 2007); doi: 10.1117/12.728344
Show Author Affiliations
Andreas Fritz, Istitute of Biomedical Optics, Univ. of Lübeck (Germany)
Lars Ptaszynski, Istitute of Biomedical Optics, Univ. of Lübeck (Germany)
Hardo Stoehr, Istitute of Biomedical Optics, Univ. of Lübeck (Germany)
Ralf Brinkmann, Istitute of Biomedical Optics, Univ. of Lübeck (Germany)


Published in SPIE Proceedings Vol. 6632:
Therapeutic Laser Applications and Laser-Tissue Interactions III
Alfred Vogel, Editor(s)

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