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Proceedings Paper

Multiphoton imaging and fluorescence lifetime studies on unstained cells and tissue at cryogenic conditions
Author(s): Martin Stark; Daniel Dörr; Alexander Ehlers; Daniel Sauer; Rainer Bückle; Sven Martin; Friederike Ehrhart; Jennifer Baunach; Alisa Katsen-Globa; Heiko Zimmermann; Karsten König
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Paper Abstract

Monitoring the functional status of cryo-preserved cells and tissue in-situ, i.e. in the frozen state, might allow for optimal adjustment of preservation conditions and might provide the information necessary to predict a functionality recovery rate. Here, an imaging approach with compositional sensitivity seems favourable. In our approach we use multiphoton microscopy in combination with fluorescence lifetime imaging to investigate cells, human and plant tissue at cryogenic conditions. By the non-linearity of multiphoton excitation we largely suppress image distortions attributed to scattering of incoming light. Only where the intensity of the pulsed near-infrared laser beam is sufficiently large, significant fluorescence is excited. This allows reaching penetration depth in ice comparable to the liquid state. As additional information we use the fluorescence decay to assign compositional entities. Results obtained on cells and tissues are discussed with respect to temperature dependencies and the related use for applications.

Paper Details

Date Published: 11 July 2007
PDF: 6 pages
Proc. SPIE 6628, Diagnostic Optical Spectroscopy in Biomedicine IV, 662809 (11 July 2007); doi: 10.1117/12.728317
Show Author Affiliations
Martin Stark, Fraunhofer Institute of Biomedical Technology (Germany)
Daniel Dörr, Fraunhofer Institute of Biomedical Technology (Germany)
Alexander Ehlers, Fraunhofer Institute of Biomedical Technology (Germany)
Univ. des Saarlandes (Germany)
Daniel Sauer, Fraunhofer Institute of Biomedical Technology (Germany)
Rainer Bückle, JenLab GmbH (Germany)
Sven Martin, JenLab GmbH (Germany)
Friederike Ehrhart, Fraunhofer Institute of Biomedical Technology (Germany)
Jennifer Baunach, Univ. des Saarlandes (Germany)
Alisa Katsen-Globa, Fraunhofer Institute of Biomedical Technology (Germany)
Heiko Zimmermann, Fraunhofer Institute of Biomedical Technology (Germany)
Univ. des Saarlandes (Germany)
Karsten König, Fraunhofer Institute of Biomedical Technology (Germany)
Univ. des Saarlandes (Germany)


Published in SPIE Proceedings Vol. 6628:
Diagnostic Optical Spectroscopy in Biomedicine IV
Dietrich Schweitzer; Maryann Fitzmaurice, Editor(s)

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