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Proceedings Paper

Investigation of depilatory mechanism by use of multiphoton fluorescent microscopy
Author(s): Chiao-Ying Lin; Gie-ne Lee; Shiou-Hwa Jee; Chen-Yuan Dong; Sung-Jan Lin
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Paper Abstract

Transdermal drug delivery provides a non-invasive route of drug administration, and can be a alternative method to oral delivery and injection. The stratum corneum (SC) of skin acts as the main barrier to transdermal drug delivery. Studies suggest that depilatory enhances permeability of drug through the epidermis. However, transdermal delivery pathway and mechanism are not completely understood. Previous studies have found that depilatory changes the keratinocytes of epidermis, and cause the protein in combination with lipid extraction of SC to become disordered. Nevertheless, those studies did not provide images of those processes. The aim of this study is to characterize the penetration enhancing effect of depilatory agent and the associated structural alterations of stratum corneum. Fresh human foreskin is treated by a depilatory agent for 10 minutes and then subjected to the treatment of fluorescent model drugs of hydrophilic rhodamine and hydrophobic rhodamine-RE. The penetration of model drugs is imaged and quantified by multiphoton microscopy. Our results showed that the penetration of both hydrophilic and hydrophobic agents can be enhanced and multifocal detachment of surface corneocytes is revealed. Nile red staining revealed, instead of a regular motar distribution of lipid around the brick of corneocytes, a disorganized and homogenized pattern of lipid distribution. We concluded that depilatory agents enhance drug penetration by disrupting both the cellular integrity of corneocytes and the regular packing of intercellular lipid of stratum corneum.

Paper Details

Date Published: 13 July 2007
PDF: 8 pages
Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 66300Z (13 July 2007); doi: 10.1117/12.728313
Show Author Affiliations
Chiao-Ying Lin, National Taiwan Univ. (Taiwan)
Gie-ne Lee, National Taiwan Univ. (Taiwan)
Shiou-Hwa Jee, National Taiwan Univ. Hospital and College of Medicine (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Sung-Jan Lin, National Taiwan Univ. (Taiwan)
Institute of Biomedical Engineering, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 6630:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging III
Tony Wilson; Ammasi Periasamy, Editor(s)

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