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Proceedings Paper

Optical projection microtomography of transparent objects
Author(s): Stanislas Vertu; Masato Ochiai; Masaki Shuzo; Ichiro Yamada; Jean-Jacques Delaunay; Olivier Haeberlé; Yoshitaka Okamoto
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Paper Abstract

We report first results in the development of a diffractive optical microtomography instrument for imaging transparent/semi-transparent biological samples without staining and sectioning. A brightfield transmission microscope was modified to form a Mach-Zehnder interferometer that was used to generate phase-shifted holograms recorded in image plane. Transparent/semi-transparent objects mixed with an index matching medium were inserted into a microcapillary and holograms of these objects were taken under different view angles by rotating the microcapillary. Precise rotation of the microcapillary was accomplished by clipping the microcapillary in a precisely machined V-groove, a system that when combined with software correction of the object centre achieved a precision of object positioning on the order of a micrometer. In this study, the observed objects were considered to be weakly diffracting and reconstructed by projection tomography of the phases of their measured scattered fields. The three-dimensional distribution of the refractive index was obtained by backprojecting the phases. Refractive index distributions are shown for a glass bead and a pollen grain. The measured difference between the refractive index of the glass bead and the microcapillary was within ±0.01. An isotropic spatial-resolution of the instrument in the micrometer range was obtained with an objective having a numerical aperture of 0.4.

Paper Details

Date Published: 11 July 2007
PDF: 6 pages
Proc. SPIE 6627, Optical Coherence Tomography and Coherence Techniques III, 66271A (11 July 2007); doi: 10.1117/12.728168
Show Author Affiliations
Stanislas Vertu, The Univ. of Tokyo (Japan)
Masato Ochiai, The Univ. of Tokyo (Japan)
Masaki Shuzo, The Univ. of Tokyo (Japan)
Ichiro Yamada, The Univ. of Tokyo (Japan)
Jean-Jacques Delaunay, The Univ. of Tokyo (Japan)
Olivier Haeberlé, Univ. of Haute Alsace (France)
Yoshitaka Okamoto, Chiba Univ. (Japan)

Published in SPIE Proceedings Vol. 6627:
Optical Coherence Tomography and Coherence Techniques III
Peter E. Andersen; Zhongping Chen, Editor(s)

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