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Proceedings Paper

Flouescence reference materials used for optical and biophotonic applications
Author(s): A. Engel; C. Otterman; J. Klahn; D. Enseling; T. Korb; U. Resch-Genger; K. Hoffmann; S. Schweizer; J. Selling; U. Kynast; F. Koberling; V. Rupertus
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Paper Abstract

Fluorescence techniques are known for their high sensitivity and are widely used as analytical tools and detection methods for product and process control, material sciences, environmental and bio-technical analysis, molecular genetics, cell biology, medical diagnostics, and drug screening. According to DIN/ISO 17025 certified standards are used for fluorescence diagnostics having the drawback of giving relative values for fluorescence intensities only. Therefore reference materials for a quantitative characterization have to be related directly to the materials under investigation. In order to evaluate these figures it is necessary to calculate absolute numbers like absorption/excitation cross sections and quantum yield. This can be done for different types of dopands in different materials like glass, glass ceramics, crystals or nano crystalline material embedded in polymer matrices. Based on the optical spectroscopy data we will discuss options for characteristic doped glasses and glass ceramics with respect to scattering and absorption regime. It has shown recently for YAG:Ce glass ceramics that for a proper determination of the quantum efficiency in these highly scattering media a reference material with similar scattering and fluorescent properties is required. This may be performed using the emission decay measurement diagnostics, where the decay time is below 100 ns. In this paper we present first results of these aspects using well performing LUMOGEN RED organic pigments for a comparison of mainly transparent glass with glass ceramics doped with various amounts of dopands e.g. ions of raw earth elements and transition metals. The LUMOGEN red is embedded in silica and polyurethane matrices. Characterisations on wavelength accuracy and lifetime for different environmental conditions (temperature, UV irradiation) have been performed. Moreover intensity patterns and results for homogeneity, isotropy, photo and thermal stability will be discussed. In a next step we will show the transfer of the characterisation methods to inorganic fluophores (YAG:Ce) in silicon. Fluorescence (steady state, decay time) and absorption (remission, absorption) spectroscopy working in different temperature regimes (10 − 350 K) are employed diagnostic methods in order to get a microscopic view of the relevant physical processes and to prove the correctness of the obtained data. The work is funded by BMBF under project number 13N8849.

Paper Details

Date Published: 11 July 2007
PDF: 9 pages
Proc. SPIE 6628, Diagnostic Optical Spectroscopy in Biomedicine IV, 662815 (11 July 2007); doi: 10.1117/12.728144
Show Author Affiliations
A. Engel, Schott AG (Germany)
C. Otterman, Schott AG (Germany)
J. Klahn, Schott AG (Germany)
D. Enseling, Schott AG (Germany)
T. Korb, Schott AG (Germany)
U. Resch-Genger, Federal Institute for Materials Research and Testing (Germany)
K. Hoffmann, Federal Institute for Materials Research and Testing (Germany)
S. Schweizer, Univ. of Paderborn (Germany)
J. Selling, Univ. of Paderborn (Germany)
U. Kynast, Univ. of Applied Science (Germany)
F. Koberling, Picoquant GmbH (Germany)
V. Rupertus, Schott AG (Germany)

Published in SPIE Proceedings Vol. 6628:
Diagnostic Optical Spectroscopy in Biomedicine IV
Dietrich Schweitzer; Maryann Fitzmaurice, Editor(s)

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