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Proceedings Paper

Characterization of silver nanoparticles deposited by an enzyme
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Paper Abstract

Based on their various interesting properties metal nanoparticles show the potential as analytical tool in electronic, optical, and catalytical applications. The different properties depending on composition, shape, and size of the single particles were utilized in many different approaches such as optics, magnetics and laser technology1. We present a way for enzymatic deposition of silver nanoparticles and a bioanalytical application in DNA microarray technology for this method. The technology consists of a microstructured chip with 10&mgr;m broad electrode gaps on the surface and specially designed readout device2. In principle we immobilize gold nanoparticle-labelled DNA in a gap between two electrodes. Afterwards a silver deposition on the bound gold nanoparticles generates a conductive layer between the electrodes. The measured drop in the resistance serves as signal for the chip-based electrical detection of DNA3. To further optimize this system the gold nanoparticles as seed are replaced by the enzyme horseradish peroxidase. For a better understanding of the enzymatically silver deposition process the formed silver particles were analyzed by spectroscopic characterization on a single particle level. Further investigations of these particles by AFM and SEM should give a hint to the connection between size/shape and the plasmonic properties at individual particles.

Paper Details

Date Published: 13 July 2007
PDF: 8 pages
Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66331V (13 July 2007); doi: 10.1117/12.728138
Show Author Affiliations
Thomas Schüler, Friedrich-Schiller-Univ. Jena (Germany)
Robert Möller, Friedrich-Schiller-Univ. Jena (Germany)
Andrea Steinbrück, Institute of Photonic Technology (Germany)
Wolfgang Fritzsche, Institute of Photonic Technology (Germany)
Jürgen Popp, Friedrich-Schiller-Univ. Jena (Germany)
Institute of Photonic Technology (Germany)


Published in SPIE Proceedings Vol. 6633:
Biophotonics 2007: Optics in Life Science
Jürgen Popp; Gert von Bally, Editor(s)

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