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Proceedings Paper

Fast and reliable identification of microorganisms by means of Raman spectroscopy
Author(s): Petra Rösch; Michaela Harz; Mario Krause; Jürgen Popp
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Paper Abstract

The identification of bacteria is necessary as fast as possible e.g. to provide an appropriate therapy for patients. Here the cultivation time should be kept to a minimum. Beside microbiological identification methods Raman spectroscopy is a valuable tool for bacteria identification. UV-resonance Raman spectroscopy enables selective monitoring of the cellular DNA/RNA content and allows for a genotaxonomic classification of the bacteria. Since UV excitation may lead to sample destruction the measurements are performed on rotated bacterial films. For a faster identification avoiding the cultivation step single bacteria analysis is necessary. Using micro-Raman spectroscopy a spatial resolution in the size range of the bacteria can be achieved. With this Raman excitation the chemical components of the whole cell are measured which leads to a phenotypic classification. For localization of bacteria inside complex matrices fluorescence labeling is achieved.

Paper Details

Date Published: 13 July 2007
PDF: 9 pages
Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66331A (13 July 2007); doi: 10.1117/12.728114
Show Author Affiliations
Petra Rösch, Friedrich-Schiller-Univ. Jena (Germany)
Michaela Harz, Friedrich-Schiller-Univ. Jena (Germany)
Mario Krause, Friedrich-Schiller-Univ. Jena (Germany)
Jürgen Popp, Friedrich-Schiller-Univ. Jena (Germany)
Institut für Photonische Technologien (Germany)


Published in SPIE Proceedings Vol. 6633:
Biophotonics 2007: Optics in Life Science
Jürgen Popp; Gert von Bally, Editor(s)

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