Share Email Print
cover

Proceedings Paper

Automated microinjection system for adherent cells
Author(s): Sachihiro Youoku; Yoshinori Suto; Moritoshi Ando; Akio Ito
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

We have developed an automated microinjection system that can handle more than 500 cells an hour. Microinjection injects foreign agents directly into cells using a micro-capillary. It can randomly introduce agents such as DNA, proteins and drugs into various types of cells. However, conventional methods require a skilled operator and suffer from low throughput. The new automated microinjection techniques we have developed consist of a Petri dish height measuring method and a capillary apex position measuring method. The dish surface height is measured by analyzing the images of cells that adhere to the dish surface. The contrast between the cell images is minimized when the focus plane of an object lens coincides with the dish surface. We have developed an optimized focus searching method with a height accuracy of ±0.2 um. The capillary apex position detection method consists of three steps: rough, middle, and precise. These steps are employed sequentially to cover capillary displacements of up to ±2 mm, and to ultimately accomplish an alignment accuracy of less than one micron. Experimental results using this system we developed show that it can introduce fluorescent material (Alexa488) into adherent cells, HEK293, with a success rate of 88.5%.

Paper Details

Date Published: 13 July 2007
PDF: 9 pages
Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66330S (13 July 2007); doi: 10.1117/12.728081
Show Author Affiliations
Sachihiro Youoku, Fujitsu Labs. Ltd. (Japan)
Yoshinori Suto, Fujitsu Labs. Ltd. (Japan)
Moritoshi Ando, Fujitsu Labs. Ltd. (Japan)
Akio Ito, Fujitsu Labs. Ltd. (Japan)


Published in SPIE Proceedings Vol. 6633:
Biophotonics 2007: Optics in Life Science
Jürgen Popp; Gert von Bally, Editor(s)

© SPIE. Terms of Use
Back to Top