Share Email Print

Proceedings Paper

Autofocus algorithms for digital-holographic microscopy
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Digital-holographic metrology enables quantitative phase contrast microscopy of reflective and (partially) transparent samples. In this way, new application fields are opened up for nondestructive investigations of technical samples as well as for marker-free and time-resolved analysis of cell biological processes. Studies on long-term biological processes require permanent focus position readjustment to maintain an optimum image quality. Digital holographic microscopy permits subsequent numerical focusing by variation of the propagation distance. Here, the determination of the optimal propagation distance for a sharply focused image is of particular importance. At the Laboratory of Biophysics image definition quantification algorithms were adapted to the requirements of digital holographic microscopy. In order to obtain robust and reliable algorithms, the object-dependent optical absorption properties were taken into consideration. Automatic focus tracking is demonstrated on investigations with digital holographic microscopy on both technical amplitude objects and cytological pure phase objects.

Paper Details

Date Published: 19 July 2007
PDF: 9 pages
Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66330E (19 July 2007); doi: 10.1117/12.727784
Show Author Affiliations
Patrik Langehanenberg, Univ. Medical Ctr., Univ. of Münster (Germany)
Björn Kemper, Univ. Medical Ctr., Univ. of Münster (Germany)
Gert von Bally, Univ. Medical Ctr., Univ. of Münster (Germany)

Published in SPIE Proceedings Vol. 6633:
Biophotonics 2007: Optics in Life Science
Jürgen Popp; Gert von Bally, Editor(s)

© SPIE. Terms of Use
Back to Top