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Proceedings Paper

Monitoring cellular metabolic pathways by wavelength- and time-resolved intracellular autofluorescence
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Paper Abstract

Autofluorescence spectroscopy has been a widely explored noninvasive technique to detect the precancerous development in epithelial tissue, where NADH and FAD fluorescence are metabolism related. In this study, we investigated the methods to monitor cellular metabolism based on the ratio of NADH over FAD fluorescence and the ratio of free NADH and protein-bound NADH fluorescence, respectively. The signals of free NADH, protein-bound NADH and FAD were isolated from the intracellular autofluorescence using wavelength- and time-resolved fluorescence spectroscopy. We demonstrated that the wavelength- and time-resolved intracellular autofluorescence can be used to monitor the cellular metabolic pathways and differentiate the normal cells from the cancer cells.

Paper Details

Date Published: 11 July 2007
PDF: 7 pages
Proc. SPIE 6628, Diagnostic Optical Spectroscopy in Biomedicine IV, 662806 (11 July 2007); doi: 10.1117/12.727610
Show Author Affiliations
Yicong Wu, Hong Kong Univ. of Science and Technology (Hong Kong China)
Wei Zheng, Hong Kong Univ. of Science and Technology (Hong Kong China)
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (Hong Kong China)


Published in SPIE Proceedings Vol. 6628:
Diagnostic Optical Spectroscopy in Biomedicine IV
Dietrich Schweitzer; Maryann Fitzmaurice, Editor(s)

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