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Proceedings Paper

In vivo multiphoton tomography: a non invasive powerful tool for biochemical investigation of human skin
Author(s): Ronan Le Harzic; Anne Colonna; Rainer Bückle; Alexander Ehlers; Christophe Hadjur; Frederic Leroy; Frederic Flament; Roland Bazin; Bertrand Piot; Iris Riemann; Karsten König
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Paper Abstract

In vivo simultaneous collagen and elastin measurements using the multiphoton tomograph DermaInspect have been performed in skin dermis. We showed it was possible to get simultaneous measurements of autofluorescence (AF) and Second Harmonic Generation (SHG) with a newly developed device using 2 PMTs for time-correlated single photon counting. Unlike elastin (AF), collagen structures are able to generate second harmonics (SHG). Comparing the images and SHG / AF ratios recorded in the depth of the outer and inner sides of the forearm of two European female volunteers (31 and 60 years old, respectively) shows differences in collagen and elastin fibres density. It decreases with depth in the 60 years old volunteer compared to the younger one, and the skin of younger volunteer shows more collagen.

Paper Details

Date Published: 23 July 2007
PDF: 7 pages
Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 66300V (23 July 2007); doi: 10.1117/12.727044
Show Author Affiliations
Ronan Le Harzic, JenLab GmbH (Germany)
Fraunhofer Institute of Biomedical Technology (Germany)
Anne Colonna, L'Oréal Recherche (France)
Rainer Bückle, JenLab GmbH (Germany)
Alexander Ehlers, Fraunhofer Institute of Biomedical Technology (Germany)
Christophe Hadjur, L'Oréal Recherche (France)
Frederic Leroy, L'Oréal Recherche (France)
Frederic Flament, L'Oréal Recherche (France)
Roland Bazin, L'Oréal Recherche (France)
Bertrand Piot, L'Oréal Recherche (France)
Iris Riemann, Fraunhofer Institute of Biomedical Technology (Germany)
Karsten König, Fraunhofer Institute of Biomedical Technology (Germany)

Published in SPIE Proceedings Vol. 6630:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging III
Tony Wilson; Ammasi Periasamy, Editor(s)

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